Inhibition of Staphylococcus aureus growth in fresh calf minced meat using low density Polyethylene films package promoted by titanium dioxide and zinc oxide nanoparticles

Marcous, Arin; Rasouli, Susan; Ardestani, Fatemeh

doi:10.22104/jpst.2017.451

Inhibition of Staphylococcus aureus growth in fresh calf minced meat using low density Polyethylene films package promoted by titanium dioxide and zinc oxide nanoparticles

Document Type : Research Article

Authors

Arin Marcous ¹

Susan Rasouli ²

Fatemeh Ardestani ³

¹ Department of Food Engineering, Sari Branch, Islamic Azad University, Sari, Iran

² Department of Nano Materials and Nano Coating, Faculty of Surface Coating and Modern Technologies, Institute for Color Science and Technology, Tehran, Iran

³ Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran

10.22104/jpst.2017.451

Abstract

Antibacterial properties of TiO₂, ZnO as well as mixed TiO₂-ZnO nanoparticles coated low density polyethylene films on Staphylococcus aureus PTCC1112 were investigated. Bactericidal efficiency of 0.5, 1 and 2 Wt% for TiO₂ and ZnO nanoparticles and also 1 Wt% mixed TiO₂-ZnO nanoparticles with TiO₂:ZnO ratios of 25:75, 50:50 and 75:25 were tested under UV and fluorescent lights exposure at two different states: films alone (Direct effect) and fresh calf minced meat packed inside the films. ZnO nanoparticle showed good antibacterial properties against Staphylococcus aureus PTCC1112. Maximum CFU reduction of 99.59% and 97.07% were obtained using 2 and 1 Wt% ZnO nanoparticle coated LDPE film under UV light for films alone as well as 62.43% and 59.57% for fresh calf minced meat packed. The best antibacterial functionalities of 96.25% and 77.11% CFU reduction were recorded for 1 Wt% TiO₂ nanoparticle coated LDPE films in the presence of UV light at direct contact with bacteria and fresh calf minced meat packed, respectively. In the case of mixed TiO₂-ZnO, maximum CFU reductions of 98.37% and 97.84% were obtained using 50:50 ratio of TiO₂: ZnO nanoparticles at the presence of UV light for direct effect and fresh calf minced meat packed, respectively. 2 Wt% ZnO nanoparticle as well as 1 Wt% mixed TiO₂-ZnO nanoparticles in ratio of 50:50 coated LDPE films were identified as the best case to improve shelf life and prevent Staphylococcus aureus PTCC1112 growth in fresh calf minced meat.

Highlights

ZnO nanoparticle showed 100% bactericidal effect against Staphylococcus aureus.
TiO₂ nanoparticle showed 96% bactericidal effect against Staphylococcus aureus.
Mixed TiO₂-ZnO showed 98% bactericidal effect against Staphylococcus aureus.

Keywords

Antibacterial agents

Fresh minced meat

LDPE films

nanoparticles

Staphylococcus aureus

[1] J. Chen, A.L. Brody, Use of active packaging structures to control the quality of a ready-to-eat meat product, Food Control. 30 (2013) 306-310.

[2] P. Swain, S.K. Nayak, A. Sasmal, T. Behera, S.K. Barik, S.K. Swain, S.S. Mishra, A.K. Sen, J.K. Das, Antimicrobial activity of metal based NPLs against microbes associated with diseases in aquaculture, World J. Microb. Biot. 30 (2014) 2491-2502.

[3]L.Ozimek,E.Pospiech,S.Narine, Nanotchenologies in food and meat processing, Acta. Sci. Pol. Technol. Aliment. 9 (2010) 401-412.

[4] R. Tankhiwale, S.K. Bajpai, Preparation, characterization and antibacterial applications of ZnO-NPLs coated polyethylene films for food packaging, Colloids. Surf. B. Biointerfaces. 90 (2012) 16-20.

[5] R.K. Raghupati, R.T. Koodali, A.C. Manna, Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide NPLs, Langmuir. 27 (2011) 4020–4028.

[6] T. Ohira, O. Yamamoto, Y. Iida, Z.E. Nakagawa, Antibacterial activity of ZnO powder with crystallographic orientation, J. Mater. Sci. -Mater. M. 19 (2008) 1407–1412.

[7] N. Padmavathy, R. Vijayaraghavan, Enhanced bioactivity of ZnO NPLs-an antibacterial study, Sci. Technol. Adv. Mat. 9 (2008) 432-438.

[8] A. Akbar, A.K. Anal, Zinc oxide NPLs loaded active packaging, a challenge study against Salmonella typhimurium and Staphylococcus aureus in ready-toeat poultry meat, Food Control. 38 (2014) 88-95.

[9] P.J.P. Espitia, N.F.F. Soares, R.F. Teofilo, J.S.R. Coimbra, D.M. Vitor, R.A. Batista, S.O. Ferreira, N.J. Andrade, A.A. Medeiros, Physical-mechanical and antimicrobial properties of nanocomposite films with pediocin and ZnO NPLs, Carbohyd. Polym. 94 (2013) 199-208.

[10] B. Panea, G. Ripoll, J. Gonzalez, A. FernandezCuello, A. Alberti, Effect of nanocomposite packaging containing different proportions of ZnO and Ag on chicken breast meat quality, J. Food. Eng. 123 (2014) 104-112.

[11] L. Zhang, Y. Jiang, Y. Ding, M. Povey, D. York, Investigation into the antibacterial behavior of suspension of ZnO NPLs (ZnO nanofluids), J. Nanopart. Res. 9 (2007) 479-489.

[12] R. Brayner, R. Ferrari-lliou, N. Brivois, S. Djediat, M. F. Benedetti, F. Fievet, Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO NPLs colloidal medium, Nano. Lett. 6 (2006) 866-870.

[13] H.M.M. Ibrahim, Photocatalytic degradation of methylene blue and inactivation of pathogenic bacteria using silver NPLs modified titanium dioxide thin films, World J. Microb. Biot. 31 (2015) 1049- 1060.

[14] C. Vacaroiu, M. Enache, M. Gartner, G. Popescu, M. Anastasescu, A. Brezeanu, N. Todorova, T. Giannakopoulou, C. Trapalis, The effect of thermal treatment on antibacterial properties of nanostructured TiO2(N) films illuminated with visible light, World J. Microb. Biot. 25 (2009) 27-31.

[15] S.H. Othman, N.R. Abd Salam, N. Zainal, R.K. Basha, R.A. Talib, Antimicrobial activity of TiO2NPL-coated film for potential food packaging applications, Int. J. Photoenerg. 2014 (2014) 1-6.

[16] R. Mehrpour, Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of positive Staphylococci- coagulase (Staphylococcus aureus and other species)-Part 3: Detection and MPN technique for low numbers, Inst. Standard. Ind. Res. Iran. 6806-3 (2006) 3-11.

[17] Y. Xing, X. Li, Z. Li, Q. Xu, Z. Che, W. Li, Y. Bai, K. Li, Effect of TiO2 NPLs on the antibacterial and physical properties of polyethylene-based film, Prog. Org. Coat. 73 (2012) 219-224.

[18] D. Prasad, C.R. Girija, A.J. Reddy, H. Nagabhushana, B.M. Nagabhushana, T.V. Venkatesha, S.T. Arun Kumar, A Study on theantibacterial activity of ZnO NPLs prepared by combustion method against E. coli, Int. J. Eng. Res. Appl. 4 (2014) 84-89.

[19] M. Fang, J.H. Chen, X.L. Xu, P.H. Yang, H.F. Hildebrand, Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests, Int. J. Antimicrob. Agents. 27 (2006) 513-517.

[20]J.R. Villalobos-Hernandez, G.G. Muller-Goymann, Sun protection enhancement of titanium dioxide crystals by the use of carnauba wax NPLs: the synergistic interaction between organic and inorganic sunscreens at nanoscale, Int. J. Pharm. 322 (2006) 161–170.

[21] P.J. Meechan, Ch. Wilson, Use of ultraviolet lights in biological safety cabinets: A contrarian view, Appl. Biosafety. 11(2006) 222-227.

[22] R.J. Watts, D. Washington, J. Howsawkeng, A.L. Teel, Comparative toxicity of hydrogen peroxide, hydroxyl radicals, and superoxide anion to Escherichia coli, Adv. Environ. Res. 7 (2003) 961- 968.

[23] N. Jones, B. Ray, R.T. Koodali, A.C. Manna, Antibacterial activity of ZnO NPLs suspensions on a broad spectrum of microorganisms, FEMS. Microbiol. Lett. 279 (2008) 71–76.

[24] G. Applerot, N. Perkas, G. Amirian, O. Girshevitz, A. Gedanken, Coating of glass with ZnO via ultrasonic irradiation and a study of its antibacterial properties, Appl. Surf. Sci. 256 (2009) 3-8

[25] R. Jalal, E.K. Goharshadi, M. Abareshi, M. Moosavi, A. Yousefi, P. Nancarrow, ZnO nanofluids: green synthesis, characterization, and antibacterial activity, Mater. Chem. Phys. 121 (2010) 198–201.

[26] Y. Xie, Y. He, P.L. Irwin, T. Jin, X. Shi, Antibacterial activity and mechanism of zinc oxide NPLs on Campylobacter jejuni, Appl. Environ. Microb. 77 (2011) 2325–2331.

[27] T. Gordon, B. Perlstein, O. Houbara, I. Felner, E. Banin, S. Margel, Synthesis and characterization of zinc/iron oxide composite NPLs and their antibacterial properties, Colloids. Surf. A. 374 (2011) 1-8.

[28] Z. Emami-Karvani, P. Chehrazi, Antibacterial activity of ZnO NPL on gram positive and gramnegative bacteria, Afr. J. Microbiol. Res. 5 (2011) 1368-1373.

[29] T. Jin, D. Sun, J. Y. Su, H. Zhang, H. J. Sue, Antimicrobial efficacy of zinc oxide quantum dots against Listeria monocytogenes, Salmonella enteritidis, and Escherichia coli O157:H7, J. Food. Sci. 74 (2009) 46-52.