Inhibition of Staphylococcus aureus growth in fresh calf minced meat using low density Polyethylene films package promoted by titanium dioxide and zinc oxide nanoparticles

Document Type : Research Article


1 Department of Food Engineering, Sari Branch, Islamic Azad University, Sari, Iran

2 Department of Nano Materials and Nano Coating, Faculty of Surface Coating and Modern Technologies, Institute for Color Science and Technology, Tehran, Iran

3 Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran


Antibacterial properties of TiO2, ZnO as well as mixed TiO2-ZnO nanoparticles coated low density polyethylene films on Staphylococcus aureus PTCC1112 were investigated. Bactericidal efficiency of 0.5, 1 and 2 Wt% for TiO2 and ZnO nanoparticles and also 1 Wt% mixed TiO2-ZnO nanoparticles with TiO2:ZnO ratios of 25:75, 50:50 and 75:25 were tested under UV and fluorescent lights exposure at two different states: films alone (Direct effect) and fresh calf minced meat packed inside the films. ZnO nanoparticle showed good antibacterial properties against Staphylococcus aureus PTCC1112. Maximum CFU reduction of 99.59% and 97.07% were obtained using 2 and 1 Wt% ZnO nanoparticle coated LDPE film under UV light for films alone as well as 62.43% and 59.57% for fresh calf minced meat packed. The best antibacterial functionalities of 96.25% and 77.11% CFU reduction were recorded for 1 Wt% TiO2 nanoparticle coated LDPE films in the presence of UV light at direct contact with bacteria and fresh calf minced meat packed, respectively. In the case of mixed TiO2-ZnO, maximum CFU reductions of 98.37% and 97.84% were obtained using 50:50 ratio of TiO2: ZnO nanoparticles at the presence of UV light for direct effect and fresh calf minced meat packed, respectively. 2 Wt% ZnO nanoparticle as well as 1 Wt% mixed TiO2-ZnO nanoparticles in ratio of 50:50 coated LDPE films were identified as the best case to improve shelf life and prevent Staphylococcus aureus PTCC1112 growth in fresh calf minced meat.


  • ZnO nanoparticle showed 100% bactericidal effect against Staphylococcus aureus.
  • TiO2 nanoparticle showed 96% bactericidal effect against Staphylococcus aureus.
  • Mixed TiO2-ZnO showed 98% bactericidal effect against Staphylococcus aureus.


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