Ultrasonic synthesis of Zn(II) methionine and ZnO nanostructures as a new precursor for ZnO nanoparticles and in-vitro study

Document Type: Research Paper


1 Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

2 Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran



Zinc(II) ions play a special role in biological systems. Methionine is a sulphur containing amino acid with IUPAC name 2-amino-4-(methylthio)butanoic acid. In this study, ultrasonic synthesis and characterization of nanostructured Zn(II) methionine (Zn-Meth) in two different solvents were investigated. The reaction of ZnCl2 and methionine ligand under ultrasonic irradiation in both methanol and DMSO leads to the formation of nano-sized Zn(II) methionine complexes. Characterization of the Zn(II) complex was performed using elemental analysis, FT-IR spectroscopy, X-ray powder diffraction (XRD), thermal gravimetry (TGA), field emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDS). The nano Zn-Meth complex, [Zn(CH3SCH2CH2CHNH2COOH)2]n, was then used as a precursor to obtaining the nano ZnO particle. An in-vitro study of the everted gut sac was also done on this complex to measure the uptake amount of zinc. The results showed that the nano-sized Zn-Meth has a higher absorption compared to its commercial and inorganic forms.

Graphical Abstract

Ultrasonic synthesis of Zn(II) methionine and ZnO nanostructures as a new precursor for ZnO nanoparticles and in-vitro study


  • A new synthetic method for the nanostructures of Zn-methionine complex by ultrasonic irradiations in two different solvents has reported.
  • ZnO nanoparticles were synthesized in 700 ºC for 2 hours under ambient atmospheric condition by using the nano Zn-methionine complex as precursor.
  • Zn-methionine nano-complex were used as zinc source to measure the absorption of it in everted sac method.


Main Subjects

[1] S.L. Vieira, Chelated minerals for poultry, Braz. J. Poultry Sci. 10 (2008) 73-79.
[2] V.R. Young, Adult amino acid requirements: the case for a major revision in current recommendations, J. Nutr. 124 (1994) 1517S-1523S.
[3] P.E. Milbury, A.C. Richer, Understanding the antioxidant controversy: Scrutinizing the "fountain of youth", Greenwood Publishing Group, 2008.
[4] M.A. Brown, J.V Thom, G.L. Orth, P. Cova, J. Juarez, Food poisoning involving zinc contamination, Arch. Environ. Health, 8 (1964) 657-660.
[5] J.A. Vinson, P. Bose, Comparison of the bioavailability of trace elements in inorganic salts, amino acid chelates and yeast, Proceedings on Mineral Elements, 80 (1981) 615–621.
[6] J. Evans, J. Richards, P. Fisher, K. Wedekind, Greater bioavailability of chelated compared with inorganic zinc in broiler chicks in the presence or absence of elevated calcium and phosphorus, Open Access Anim. Physiol. 7 (2015) 97-110.
[7] H.D. Ashmead, Amino acid chelation in human and animal nutrition, CRC Press, 2012.
[8] M.A. Mamun, O. Ahmed, P.K. Bakshi, M.Q. Ehsan, Synthesis and spectroscopic, magnetic and cyclic voltammetric characterization of some metal complexes of methionine: [(C5H10NO2S)2MII]; MII=Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II), J. Saudi Chem. Soc. 14 (2010) 23-31.
[9] F.H.A. Al-Jeboori, T.A.M. Al-Shimiesawi, O.M.N. Jassim, Synthesis and characterization of some essential amino acid metal complexes having biological activity, J. Chem. Pharm. Res. 5 (2013) 172-176.
[10] L.-K. Wang, L. Li, X.-M. Li, Y.-H. Shi, L. Hu, G.-W. Le, Microwave assisted solid state reaction synthesis of methionine complexes of iron (II), Food Chem. 106 (2008) 315-323.
[11] Y. Yin, A.P. Alivisatos, Colloidal nanocrystal synthesis and the organic-inorganic interface, Nature, 437 (2005) 664-670.
[12] V. Mohammadi, S. Ghazanfari, A. Mohammadi-Sangcheshmeh, M.H. Nazaran, Comparative effects of zinc-nano complexes, zinc-sulphate and zinc-methionine on performance in broiler chickens, Braz. J. Poultry. Sci. 56 (2015) 486-493.
[13] M.H. Nazaran, "Chelate compounds" U.S. Patent No. 8,288,587 B2 (issued Apr. 26, 2012).

[14] V. Safarifard, A. Morsali, Applications of ultrasound to the synthesis of nanoscale metal-organic coordination polymers, Coordin. Chem. Rev. 292 (2015) 1-14.
[15] M. Asokkumar, T.J. Mason, Sonochemistry. Kirk-Othmer Encyclopedia of Chemical Technology. John Wiley and Sons Inc, 2007.
[16] M. Ranjbar, N. Shahsavan, M. Yousefi, Synthesis and characterization of nanostructured zinc (II) cysteine complex under ultrasound irradiation, Amer. Chem. Sci. J. 2 (2012) 111-121.
[17] K.S. Sindhura, T. Prasad, P.P. Selvam, O.M. Hussain, Synthesis, characterization and evaluation of effect of phytogenic zinc nanoparticles on soil exo-enzymes, Appl. Nanosci. 4 (2014) 819-827.
[18] J.F. Hillyer, R.M. Albrecht, Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles, J. Pharm. Sci. 90 (2001) 1927-1936.
[19] Y.B.Z. Hongfu, Effects of nano-ZnO on growth performance and diarrhea rate in weaning piglets, China Feed. 1(2008) 8.
[20] C.J. Zhisheng, Effect of nano-zinc oxide supplementation on rumen fermentation in-vitro, Chinese J. Anim. Nutr, 8 (2011) 23.
[21] T. Lina, J. Jianyang, Z. Fenghua, R. Huiying, L. Wenli, Effect of nano-zinc oxide on the production and dressing performance of broiler, Chinese Agric. Sci. Bull. 2 (2009) 3.
[22] V. Patsula, M. Moskvin, S. Dutz, D. Horák, Size-dependent magnetic properties of iron oxide nanoparticles, J. Phys. Chem. Solid. 88 (2016) 24-30.
[23] J. Robertson, High dielectric constant gate oxides for metal oxide Si transistors, Reports Prog. Phys. 69 (2005) 327.
[24] A.V. Emeline, G.V. Kataeva, A.V. Panasuk, V.K. Ryabchuk, N.V. Sheremetyeva, N. Serpone, Effect of surface photoreactions on the photocoloration of a wide band gap metal oxide: probing whether surface reactions are photocatalytic, J. Phys. Chem. B. 109 (2005) 5175-5185.
[25] M.A. Alam, F.I. Al-Jenoobi, A.M. Al-Mohizea, F.I. Al-Jenoobi, A.M. Al-Mohizea, Everted gut sac model as a tool in pharmaceutical research: limitations and applications, J. Pharm. Pharmacol. 64 (2012) 326-336.
[26] M.R. Mahmoud, Everted intestinal sacs as in-vitro model for assessing absorptivity of L-histidine under the effect of aspirine and gum acacia in male rats, Egypt. J. Hosp. Med. 16 (2004) 14-28.

[27] S. Furuta, S. Toyama, H. Sano, Absorption mechanism of polaprezinc (zinc l-carnosine complex) by an everted sac method, Xenobiotica, 24 (1994) 1085-1094.
[28] R.B. Wilson, P. De Meester, D.J. Hodgson, Structural characterization of bis(L-methionato) zinc (II), Zn(L-Met)2, Inorg. Chem. 16 (1977) 1498-1502.
[29] B. Evertsson, The crystal structure of bis-L-histidine copper(II) dinitrate dihydrate, Acta Crystallogr. Sect. B, 25 (1969) 30-41.

[30] F. Ji, X.G. Luo, L. Lu, B. Liu, S.X. Yu, Effects of manganese source and calcium on manganese uptake by in vitro everted gut sacs of broilers’ intestinal segments., Poult. Sci. 85 (2006) 1217-1225.
[31] L. Zhang, F. Gu, J. Chan, A. Wang, R. Langer, O. Farokhzad, Nanoparticles in medicine: Therapeutic applications and developments, Clin. Pharmacol. Ther. 83 (2008) 761-769.